Application of Factorial Design for the Optimization of Pleurotus Afin Ostreatus Production Using Grass As Substrate

The organic residue from gardens, which is generally not used efficiently, has been the central focus of this research work. That why present work factorial methodology in optimization production Pleurotus afín ostreatus using an residue, with primary objective as a substrate, adjusting it through balance C/N ratio, for specific cultivation Pleurotus. This one edible mushroom species worldwide, known its mycelial biomass and bioactive components. comprised several stages, including propagation fungus, generation seeds barley grains, inoculation 5% inoculum polypropylene bags containing four different types substrates, five replicates each variant. resulting was measured, nutritional analysis fruiting bodies obtained substrate highest productivity carried out. results parameters responses afin on by ANOVA method were (p<0.05) F-values models factor A (type substrate), B crop) AB 6.78, 2.99 6.10, respectively. composition determined. These exhibited considerable water content, reached 75.44%. On other hand, low concentration carbohydrates observed, value 15.86%, minimal amount fats, barely 0.13%. It important to note that there particularly notable protein 8.09% relation wet weight. Finally, concluded values indicate best growth fungus S3, whose biological efficiency among recorded (37.849%) fungus. In addition, means design, observed order have greater productive capacity ostreatus, necessary ratio 40.13C/N type sampling per day mass 77g.